Carbohydrate Moieties and Cytoenzymatic Characterization of Hemocytes in Whiteleg Shrimp Litopenaeus vannamei

نویسندگان

  • Norma Estrada
  • Edwin Velázquez
  • Carmen Rodríguez-Jaramillo
  • Felipe Ascencio
چکیده

Hemocytes represent one of the most important defense mechanisms against foreign material in Crustacea and are also involved in a variety of other physiological responses. Fluorescent lectin-binding assays and cytochemical reactions were used to identify specificity and distribution of carbohydrate moieties and presence of several hydrolytic enzymes, in hemocytes of whiteleg shrimp Litopenaeus vannamei. Two general classes of circulating hemocytes (granular and agranular) exist in L. vannamei, which express carbohydrates residues for FITC-conjugated lectins WGA, LEA, and PNA; UEA and Con-A were not observed. Enzymatic studies indicated that acid phosphatase, nonspecific esterase, and specific esterases were present; alkaline phosphatase was not observed. The enzymes and carbohydrates are useful tools in hemocyte classification and cellular defense mechanism studies.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

The effect of different levels of dietary niacin and carbohydrate on growth performance, carcass composition and heamolymph biochemical parameters of juvenile whiteleg shrimp (Litopenaeus vannamei)

In this study, the effect of experimental diets containing different levels of carbohydrate and niacin on growth indices, carcass composition and heamolymph biochemical parameters of juvenile whiteleg shrimp (Litopenaeus vannamei) were investigated for 45 days. Shrimp with mean initial weight of 5±0.33 g were reared in 300 liter tanks with 8 treatments. Feeding was performed 4 times a day using...

متن کامل

Apparent carbohydrate and lipid digestibility of feeds for whiteleg shrimp, Litopenaeus vannamei (Decapoda: Penaeidae), cultivated at different salinities.

Whiteleg shrimp, Litopenaeus vannamei is one of the most commercially farmed species worldwide because of its fast growth, good survival rate at high farming densities, and osmoregulatory capacity, which makes it an excellent candidate for cultures at different salinities. The knowledge of shrimp nutritional requirements is critical in the formulation of diets to allow optimal growth at differe...

متن کامل

The effects of different concentrations of selenium and zinc nanoparticles on growth performance, survival and chemical composition of Whiteleg shrimp (Litopenaeus vannamei)

This study was conducted to evaluate the effect of different concentrations of selenium and zinc nanoparticles on growth performance, survival and chemical composition of Whiteleg shrimp (Litopenaeus vannamei). For this purpose, shrimp with an average weight of 5.1±0.2 g were distributed in 12 fiberglass tanks with 25 shrimp density and were fed for 56 days with different concentrations of sele...

متن کامل

Using date pits powder as a low-cost carbohydrate ingredient in the diet of whiteleg shrimp Penaeus vannamei

The present study examined the effect of date pits powder as a low-cost and nutritive carbohydrate source compared to wheat, rice and corn meal in the diet of Pacific shrimp. The date pits meal was applied to the diet at the levels of 0 (control group), 5, 10, 15 and 23%, and the growth performance of shrimp was examined in 8 weeks. The results showed that the weight and length gain, specific g...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:

دوره 2016  شماره 

صفحات  -

تاریخ انتشار 2016